Alteration in the Distribution of Basic Soluble Rat Liver Proteins during Azo Dye Carcinogenesis1
نویسندگان
چکیده
Antibodies against rat liver // proteins and against a similar charge class of azoproteins have been labeled with fluorescein isothiocyanate (FITC), and the labeled antisera have been used as histoserological stains on unfixed frozen sections of rat liver. Fixation of antibody was defined by fluorescence microscopy and was used to determine the intracellular distribution of the appropriate antigens. FITC-labeled anti-/; protein stained the cytoplasm of both normal and 3'-methyl-4-dimethylaminoazobenzene (3'MeDAB)-treated rat livers uniformly but did not stain 3'MeDAB-induced rat
منابع مشابه
Alteration in the distribution of basic soluble rat liver proteins during azo dye carcinogenesis.
Antibodies against rat liver // proteins and against a similar charge class of azoproteins have been labeled with fluorescein isothiocyanate (FITC), and the labeled antisera have been used as histoserological stains on unfixed frozen sections of rat liver. Fixation of antibody was defined by fluorescence microscopy and was used to determine the intracellular distribution of the appropriate anti...
متن کاملZonal Electrophoresis of the Soluble Proteins of Liver and Tumor in Azo Dye Carcinogenesis.
A procedure has been developed for the zonal electrophoretic resolution of the sol uble proteins of rat liver with the use of a modified Porath column of ethanolyzed cellu lose. The mild method, with 750 mg. on less of protein, has been applied to various studies. An initial degree of resolution reproducibly separates the soluble liver proteins into all their principal charge classes with high ...
متن کاملBile Acid Involvement in Azo Dye Carcinogenesis1
the early stages of liver carcinoma development in Sprague-Dawley rats. Bile acids were analyzed in urine and feces of azo dye-fed and control rats with the use of gas chromatography during the first 66 days of treatment. Seven bile acids were identified in the feces, with deoxycholic acid being the primary excretion product. During the first 36 days of 3'-methyl-4-dimethylaminoazobenzene feedi...
متن کاملEarly studies on hepatocarcinogenesis induced by amino azo dyes in the rat revealed an increase in DNA and a decrease in RNA per gm tissue in precancerous liver and hepatoma (11, 12), although occasional inconsistencies
azo dyes in the rat revealed an increase in DNA and a decrease in RNA per gm tissue in precancerous liver and hepatoma (11, 12), although occasional inconsistencies were observed. Recent studies (9) in which RNA and DNA were isolated and purified have confirmed the de crease in total RNA relative to DNA. In these studies O'Sullivan (9) found no significant alteration in nucleotide base composit...
متن کاملIrreversible fixation of increased level of muscle type aldolase activity appearing in rat liver in the early stage of hepatocarcinogenesis.
azobenzene for 60 days. The muscle type aldolase in the livers of rats thus treated was found to be elevated when compared to nor mal liver, although no appreciable differences were ob served in total aldolase activity, mitotic index, histological architecture, and soluble protein, as well as DNA content. The appearance of this enzymic change was already observable by administrating the azo dye...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2006